کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1430797 | 1509192 | 2006 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Impedance spectroscopy and affinity measurement of specific antibody–antigen interaction
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی مواد
بیومتریال
پیش نمایش صفحه اول مقاله
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چکیده انگلیسی
We use impedance spectroscopy technique to develop a rapid and sensitive antigen biosensor. The biosensor is based on the immobilization of antibodies onto an 11-Mercaptoundecanoic acid self-assembled monolayer. The high insulating properties of the thiol can be measured with cyclic voltammetry and impedance spectroscopy with a redox couple. The, the modified gold electrode is activated with 1-ethyl-3 (3-dimethyl aminopropyl) carbodiimide (EDC) and N-hydroxy succinimide (NHS) ester for antibody immobilisation. The affinity interaction of the antigen–antibody can be measured with quartz crystal microbalance technique. Dissociation and association constant are determined by applying a Fortran program fitting for the experimental data.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Materials Science and Engineering: C - Volume 26, Issues 2–3, March 2006, Pages 546–550
Journal: Materials Science and Engineering: C - Volume 26, Issues 2–3, March 2006, Pages 546–550
نویسندگان
A. Tlili, A. Abdelghani, S. Ameur, N. Jaffrezic-Renault,