Biosynthesis of gold nanoparticles by biosorption using Magnetospirillum gryphiswaldense MSR-1

The biosynthesis of nanoparticles is considered to be a novel, effective and eco-friendly procedure. In this paper, Magnetospirillum gryphiswaldense MSR-1 was successfully used to reduce gold ions to a zero-valent metal in a water environment, and more importantly, to accumulate them into spherical nanoparticles on the cell surface through biosorption. Transmission electron microscopy (TEM) was used to examine nanoparticle characteristics. The size range of the nano-gold made by MSR-1 was from 10 to 40 nm. X-ray diffraction (XRD) and X-ray photoelectron spectroscopy (XPS) analyses of the gold nanoparticles confirmed the formation of metallic gold. The pH value of the biosynthesis solution and the initial metal concentration were examined; both of these important parameters were isolated and screened to determine their role in controlling particle size. The results demonstrated that lower pH values and initial concentrations of Au(III) were individually responsible for reductions in nano-gold particle size.

► Magnetospirillum gryphiswaldense MSR-1 was successfully used in reducing gold ions into nulvalent metal in water environment.
► Accumulate the numvalent metal in spherical nanoparticles onto the cell surface through biosorption.
► pH value and initial concentration of Au(III) influenced the size of gold nanoparticles.
► Lower the pH value and the initial concentration of Au(III), the smaller the nano-gold particle size.