Homogeneous and reproducible liposome preparation relying on reassembly in microchannel laminar flow

We investigated a method for size homogenization of liposomes using microchannel laminar flow. This microchannel method combined with sonication produced the desired homogeneous liposome populations with size controlled by reassembly of liposomes in laminar flow, using simple operations that offer good reproducibility and organic-solvent free procedures. The liposome solution, which was prepared using the traditional method of film hydration, was loaded into a syringe. This liposome solution was sonicated while being transported into the capillary tubing using syringe injection. In both non-sonicated and sonicated batchwise preparations, liposomes displayed non-homogeneous and non-reproducible size profiles. On the other hand, homogeneous liposomes were obtained with good reproducibility using our microchannel method combined with sonication.