کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
152433 456495 2010 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effect of diffusion on enzyme activity in a microreactor
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی مهندسی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
Effect of diffusion on enzyme activity in a microreactor
چکیده انگلیسی

To establish general rules for setting up an enzyme microreactor system, we studied the effect of diffusion on enzyme activity in a microreactor. As a model system we used the hydrolysis of ortho-nitrophenyl-β-d-galactopyranoside by β-galactosidase from Kluyveromyces lactis. We found that the Michaelis–Menten kinetic parameters were similar at the microscale and bench scale. With residence times below a few seconds, diffusion effects limited the reaction rate and therefore reduced the conversion per volume of enzyme microreactor. The critical residence time where diffusion limits the conversion increased quadratically with channel width, increased with enzyme concentration, and decreased with substrate concentration. These general rules can be used for choosing parameters when setting up an enzyme microreactor system. To use an enzyme microreactor efficiently, diffusion effects should be taken into account.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chemical Engineering Journal - Volume 162, Issue 1, 1 August 2010, Pages 301–306
نویسندگان
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