In silico analysis of cis-acting regulatory elements in 5′ regulatory regions of sucrose transporter gene families in rice (Oryza sativa Japonica) and Arabidopsis thaliana

The regulation of gene expression involves a multifarious regulatory system. Each gene contains a unique combination of cis-acting regulatory sequence elements in the 5′ regulatory region that determines its temporal and spatial expression. Cis-acting regulatory elements are essential transcriptional gene regulatory units; they control many biological processes and stress responses. Thus a full understanding of the transcriptional gene regulation system will depend on successful functional analyses of cis-acting elements.Cis-acting regulatory elements present within the 5′ regulatory region of the sucrose transporter gene families in rice (Oryza sativa Japonica cultivar-group) and Arabidopsis thaliana, were identified using a bioinformatics approach.The possible cis-acting regulatory elements were predicted by scanning 1.5 kbp of 5′ regulatory regions of the sucrose transporter genes translational start sites, using Plant CARE, PLACE and Genomatix Matinspector professional databases.Several cis-acting regulatory elements that are associated with plant development, plant hormonal regulation and stress response were identified, and were present in varying frequencies within the 1.5 kbp of 5′ regulatory region, among which are; A-box, RY, CAT, Pyrimidine-box, Sucrose-box, ABRE, ARF, ERE, GARE, Me-JA, ARE, DRE, GA-motif, GATA, GT-1, MYC, MYB, W-box, and I-box. This result reveals the probable cis-acting regulatory elements that possibly are involved in the expression and regulation of sucrose transporter gene families in rice and Arabidopsis thaliana during cellular development or environmental stress conditions.

Figure optionsDownload as PowerPoint slideResearch highlights▶ Developmental, hormonal & stress response regulatory elements were identified. ▶ All rice and Arabidopsis sucrose transporters possibly involved in abiotic stress. ▶ OsSUT2, 4 & 5 and AtSUC6 & 9 possibly involved in cellular development. ▶ OsSUT2 & 3 and AtSUC2, 3, 5, 6 & 8 possibly involved in biotic stress. ▶ All rice and Arabidopsis sucrose transporters regulated at a low level by hormones.