کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1627908 | 1516461 | 2006 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Application of cerium(IV)/EDTA complex for future biotechnology
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موضوعات مرتبط
مهندسی و علوم پایه
مهندسی مواد
فلزات و آلیاژها
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چکیده انگلیسی
A new artificial system for site-selective hydrolysis of single-stranded DNAs was prepared. By using two oligonucleotide additives that bear a monophosphate group at the termini, gap structures were formed at predetermined positions in substrate DNA. The phosphodiester linkages in the gap were efficiently and selectively hydrolyzed by Ce(IV)/EDTA complex (EDTA, ethylenediamine-N,N,Nâ²,Nâ²-tetraacetate) at pH 7.0 and 37 °C. Furthermore, the fragments formed by the site-selective scission were connected with various oligonucleotides by using T4 DNA ligase, producing desired recombinant DNAs. A new tool for manipulation of single-stranded DNA in biotechnology has been successfully obtained.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Alloys and Compounds - Volumes 408â412, 9 February 2006, Pages 391-395
Journal: Journal of Alloys and Compounds - Volumes 408â412, 9 February 2006, Pages 391-395
نویسندگان
Jun Sumaoka, Wen Chen, Yoshihito Kitamura, Takafumi Tomita, Junya Yoshida, Makoto Komiyama,