Quantitative characterization of the electrospun gelatin-chitosan nanofibers by coupling scanning electron microscopy and atomic force microscopy

► Nanofibers were electrospun from gelatin and chitosan at different mass ratios. ► Nanofibers were quantitatively characterized by SEM and AFM-HarmoniX mode. ► The gelatin-chitosan samples showed more uniform morphologies than the pure ones. ► The gelatin-chitosan samples showed higher DMT modulus values than the pure ones. ► DMT modulus indicated intermolecular interaction in gelatin-chitosan nanofibers.