کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
16899 | 42621 | 2016 | 7 صفحه PDF | دانلود رایگان |
• Here reports a convenient, efficient, one-step synthesis of palmitanilide under biocatalysis.
• This is an economic and environment friendly process to produce the palmitanilide.
• The obtained results of kinetic study are valuable for elucidating the reaction mechanism and represent.
• An important contribution for reaction optimization and creating strategies to increase the productivity of amide synthesis.
• The synthesized palmitanilide shows antibacterial activity against B. cereus and is potentially an antibacterial agent.
Enzymatic syntheses of fatty acid anilides are important owing to their wide range of industrial applications in detergents, shampoo, cosmetics, and surfactant formulations. The amidation reaction of Mucor miehei lipase Lipozyme IM20 was investigated for direct amidation of triacylglycerol in organic solvents. The process parameters (reaction temperature, substrate molar ratio, enzyme amount) were optimized to achieve the highest yield of anilide. The maximum yield of palmitanilide (88.9%) was achieved after 24 h of reaction at 40 °C at an enzyme concentration of 1.4% (70 mg). Kinetics of lipase-catalyzed amidation of aniline with tripalmitin has been investigated. The reaction rate could be described in terms of the Michaelis–Menten equation with a Ping–Pong Bi–Bi mechanism and competitive inhibition by both the substrates. The kinetic constants were estimated by using non-linear regression method using enzyme kinetic modules. The enzyme operational stability study showed that Lipozyme IM20 retained 38.1% of the initial activity for the synthesis of palmitanilide (even after repeated use for 48 h). Palmitanilide, a fatty acid amide, exhibited potent antimicrobial activity toward Bacillus cereus.
Figure optionsDownload as PowerPoint slide
Journal: Enzyme and Microbial Technology - Volume 82, January 2016, Pages 82–88