کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1696553 | 1519134 | 2007 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Optimum immobilization of Candida rugosa lipase on Celite by RSM Optimum immobilization of Candida rugosa lipase on Celite by RSM](/preview/png/1696553.png)
Immobilization of Candida rugosa lipase (lipase AY-30) by adsorption on Celite led to a markedly improved performance of the enzyme. Immobilization conditions and characterization of the immobilized enzyme were investigated. Lipase activity was measured with glycerol tributyrate as substrate. Response surface methodology (RSM) and 3-level–3-factor fractional factorial design were employed to evaluate the effects of immobilization parameters, such as immobilization time (30–90 min), immobilization temperature (0–20 °C), and enzyme/support ratio (0.3–0.5, w/w), on the specific activity of immobilized lipase. Based on the analysis of ridge max, the optimum immobilization conditions were as follows: immobilization time 59.1 min, immobilization temperature 10.7 °C, and enzyme/support ratio 0.5 (w/w); the highest specific activity obtained was 18.16 U/mg-protein with activity yield of 34.1%.
Journal: Applied Clay Science - Volume 37, Issues 1–2, June 2007, Pages 67–73