Immobilization of horseradish peroxidase in phospholipid-templated titania and its applications in phenolic compounds and dye removal

• HRP was encapsulated in titania particles through biomimetic titanification process.
• The stabilities of HRP were obviously improved by encapsulation in titania.
• The encapsulated HRP can be used in removal of dye and phenolic compounds.
• The encapsulated HRP showed better removal efficiency than free HRP.
• The encapsulated HRP showed a moderately good capability of reutilization.

In this study, horseradish peroxidase (HRP) was encapsulated in phospholipid-templated titania particles through the biomimetic titanification process and used for the treatment of wastewater polluted with phenolic compounds and dye. The encapsulated HRP exhibited improved thermal stability, a wide range of pH stability and high tolerance against inactivating agents. It was observed an increase in Km value for the encapsulated HRP (8.21 mM) when compared with its free counterpart. For practical applications in the removal of phenolic compounds and dye by the encapsulated HRP, the removal efficiency for phenol, 2-chlorophenol, Direct Black-38 were 92.99%, 87.97%, and 79.72%, respectively, in the first treatment cycle. Additionally, the encapsulated HRP showed better removal efficiency than free HRP and a moderately good capability of reutilization.