CFD modelling of two-phase stirred bioreaction systems by segregated solution of the Euler–Euler model

An advanced study of a bioreactor system involving a Navier–Stokes based model has been accomplished. The model allows a more realistic impeller induced flow image to be combined with the Monod bioreaction kinetics reported previously. The time-course of gluconic acid production by Aspergillus niger strain is simulated at kinetic conditions proposed in the literature. The simulation is based on (1) a stepwise solution strategy resolving first the fluid flow field, further imposing oxygen mass transfer and bioreaction with subsequent analysis of flow interactions, and (2) a segregated solution of the model replacing the multiple iterations per grid cell with single iterations. The numerical results are compared with experimental data for the bioreaction dynamics and show satisfactory agreement. The model is used for assessment of the viscosity effect upon the bioreactor performance. A 10-fold viscosity rise results in 2-fold decrease of KLa and 25% decrease of the specific gluconic acid production rate. The model allows better understanding of the mechanism of the important bioprocess.

► A RANS flow model combined with Monod kinetic allow improved bioprocess simulation.
► Euler–Euler is solved by segregation of multiple to single iterations per grid cell.
► The example system is microbial gluconic acid production.
► A 10-fold viscosity rise leads to 25% reduction of gluconic acid production rate.