کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1746314 | 1522228 | 2010 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Utilisation of phosphorus nutrient content in industrial scale plasmid DNA production: a waste minimisation study
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی انرژی
انرژی های تجدید پذیر، توسعه پایدار و محیط زیست
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چکیده انگلیسی
Human gene therapy is currently seeing an increase in the use of plasmid (pDNA)-based vectors as a preferred choice of vehicle for delivery of the therapeutic gene into the body. In this regard, the environmental impacts of the waste streams from the possible industrial scale manufacture of pDNA require more detailed assessment. In this study, an initial assessment was made of the nutrient phosphorus (P) inputs to four fermentation processes recommended for the industrial scale production of pDNA for the purposes of gene therapy. Phosphorus inputs to each of the four selected fermentation processes ranged from approximately 60 mg lâ1 up to 3000 mg lâ1 in the fresh media. However, the spent media waste from each of the processes exhibited only a minor reduction in the phosphorus concentrations indicating minimal uptake of P by the microorganisms. This unutilised excess level of phosphorus nutrient within the waste streams poses a strong potential for environmental impact. Waste minimisation studies were undertaken on one model fermentation process with the aim of reducing unnecessary phosphorus input. An optimised media containing a 98% reduction in added P to the media was developed. This phosphorus-minimised media had little quantitative effect upon cell biomass produced and no effect upon the quantity or quality of pDNA produced, relative to the control media. The reduction in P requirement results in an overall cost savings of 12% per fermentation batch, would simplify subsequent wastewater treatment and would contribute to slowing the depletion rate of a valuable, finite natural resource.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Cleaner Production - Volume 18, Issues 10â11, July 2010, Pages 1066-1072
Journal: Journal of Cleaner Production - Volume 18, Issues 10â11, July 2010, Pages 1066-1072
نویسندگان
Finola E. Cliffe, Gary Walsh, Thomas F. O'Dwyer,