High Frequency Ultrasound Imaging Detects Cardiac Dyssynchrony in Noninfarcted Regions of the Murine Left Ventricle Late After Reperfused Myocardial Infarction

Cardiac dyssynchrony in the left ventricles of murine hearts late (≥28 d) after reperfused myocardial infarction (post-MI) was assessed using high frequency 30 MHz B-mode ultrasound imaging. Nine post-MI and six normal C57Bl/6 mice were studied in both short- and long-axis views. Regional time to peak displacement (Tpeak_d) and time to peak strain (Tpeak_s) were calculated in 36 sectors along the myocardial circumference; then their standard deviations (SD_Tpeak_d and SD_Tpeak_s) were computed among noninfarcted myocardial regions for each mouse and were compared between the normal and post-MI mouse groups with Student's t-test. The comparison revealed that SD_Tpeak_d and SD_Tpeak_s were significantly larger in the post-MI hearts than in the normal hearts. The displacement uniformity ratio was determined to be 0.97 ± 0.01 and 0.85 ± 0.07 for radial and circumferential displacements in the normal hearts, respectively; and 0.59 ± 0.17 and 0.64 ± 0.24 in the post-MI hearts. In conclusion, this high resolution ultrasound image tracking method provides for the detection of cardiac dyssynchrony in the noninfarcted regions in the murine left ventricles late after MI by identifying the temporal and spatial disparity of regional myocardial contraction. (E-mail: jh7fj@virginia.edu)