کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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17648 | 42686 | 2010 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Characterization of d-galactosyl-β1→4-l-rhamnose phosphorylase from Opitutus terrae Characterization of d-galactosyl-β1→4-l-rhamnose phosphorylase from Opitutus terrae](/preview/png/17648.png)
We characterized a glycoside hydrolase family 112 protein from Opitutus terrae (Oter_1377 protein). The enzyme phosphorolyzed d-galactosyl-β1→4-l-rhamnose (GalRha) and also showed phosphorolytic activity on d-galactosyl-β1→3-d-glucose as a minor substrate. In the reverse reaction, the enzyme showed higher activity on l-rhamnose derivatives than on d-glucose derivatives. The enzyme was stable up to 45 °C and at pH 6.0–7.0. The values of kcat and Km of the phosphorolytic activity of the enzyme on GalRha were 60 s−1 and 2.1 mM, respectively. Thus, Oter_1377 protein was identified as d-galactosyl-β1→4-l-rhamnose phosphorylase (GalRhaP). The presence of GalRhaP in O. terrae suggests that genes encoding GalRhaP are widely distributed in different organisms.
Journal: Enzyme and Microbial Technology - Volume 46, Issues 3–4, 5 March 2010, Pages 315–319