کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
17907 | 42704 | 2007 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A dual phase fermentation protocol for the production of hydantoinase and carbamoylase by the wild type Pseudomonas putida RU-KM3
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موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
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چکیده انگلیسی
The fermentation of the hydantoinase-active Pseudomonas putida RU-KM3s was investigated. The hydantoinase activity was found to be reduced in complex media, while the use of high sugar to nitrogen ratios in the growth medium resulted in the formation of extracellular polymers, low biomass, and low enzyme activity. By replacing the primary carbon source from simple carbohydrate to a fatty acid, using an ammonia-deficient medium (designated PP2 medium), a maximum growth rate of 0.29 (±0.005) hâ1 was attained with a biomass of 7.3 (±0.18) g Lâ1 at a yield of 0.73 g gâ1 and a productivity of 0.61 g gâ1 hâ1. The maximum hydantoinase activity obtained was 187 (±19.4) nmol minâ1 mgâ1 (DCW), while that of the N-carbamoylase was 22 (±3.1) nmol minâ1 mgâ1 (DCW). The fermentation was scaled up 50-fold from shake flask to 15 L batch.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Enzyme and Microbial Technology - Volume 41, Issue 4, 3 September 2007, Pages 539-545
Journal: Enzyme and Microbial Technology - Volume 41, Issue 4, 3 September 2007, Pages 539-545
نویسندگان
Shaun Kirchmann, Petrus van Zyl, Dean Brady, Nasreen Abrahams, Sonia Rech, Rosemary Dorrington, Stephanie Burton,