کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
17907 42704 2007 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A dual phase fermentation protocol for the production of hydantoinase and carbamoylase by the wild type Pseudomonas putida RU-KM3
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
A dual phase fermentation protocol for the production of hydantoinase and carbamoylase by the wild type Pseudomonas putida RU-KM3
چکیده انگلیسی
The fermentation of the hydantoinase-active Pseudomonas putida RU-KM3s was investigated. The hydantoinase activity was found to be reduced in complex media, while the use of high sugar to nitrogen ratios in the growth medium resulted in the formation of extracellular polymers, low biomass, and low enzyme activity. By replacing the primary carbon source from simple carbohydrate to a fatty acid, using an ammonia-deficient medium (designated PP2 medium), a maximum growth rate of 0.29 (±0.005) h−1 was attained with a biomass of 7.3 (±0.18) g L−1 at a yield of 0.73 g g−1 and a productivity of 0.61 g g−1 h−1. The maximum hydantoinase activity obtained was 187 (±19.4) nmol min−1 mg−1 (DCW), while that of the N-carbamoylase was 22 (±3.1) nmol min−1 mg−1 (DCW). The fermentation was scaled up 50-fold from shake flask to 15 L batch.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Enzyme and Microbial Technology - Volume 41, Issue 4, 3 September 2007, Pages 539-545
نویسندگان
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