کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
17937 42706 2008 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Bicontinuous microemulsion as reaction medium for the β-glucosidase-catalyzed synthesis of n-hexyl-β-d-glucopyranoside
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Bicontinuous microemulsion as reaction medium for the β-glucosidase-catalyzed synthesis of n-hexyl-β-d-glucopyranoside
چکیده انگلیسی

n-Hexyl-β-d-glucopyranoside (HGP) was synthesized through the β-glucosidase-catalyzed condensation of glucose and hexanol in an aqueous-organic bicontinuous microemulsion system with both water and oil as continuous phases. Methodology for separation of HGP from the microemulsion system for analysis by gas chromatography was developed. Various factors influencing the synthesis of HGP like reaction time, enzyme concentration and temperature were optimized experimentally. β-Glucosidase (1.1 U/ml) yielded a final HGP concentration of 6.5 mM at 45 °C after 24 h reaction. Reverse reaction (hydrolysis) occurred was also studied. Comparison of the rate of the reaction between synthesis and hydrolysis showed the latter to be faster, suggesting that bicontinuous microemulsion system is more applicable to the enzymatic hydrolysis. Degradation of enzyme in bicontinuous microemulsion system was very negligible.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Enzyme and Microbial Technology - Volume 42, Issue 3, 4 February 2008, Pages 252–258
نویسندگان
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