کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
18166 42713 2007 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Two novel surfactant-stable alkaline proteases from Vibrio fluvialis TKU005 and their applications
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Two novel surfactant-stable alkaline proteases from Vibrio fluvialis TKU005 and their applications
چکیده انگلیسی

An alkaliphilic protease-producing bacterium was isolated and identified as Vibrio fluvialis TKU005. Two surfactant-stable alkaline metalloproteases (FI and FII) were purified from the culture supernatant of shrimp shell powder containing medium (pH 10). The molecular masses of FI and FII were 41 kDa and 39 kDa, respectively, assayed by SDS-PAGE. The optimum pH, optimum temperature, pH stability, thermal stability, Km, and Vmax of FI and FII were (9, 60 °C, 7–9, 50 °C, 2.21 mg/mL, 0.26 U/mL) and (9, 60 °C, 6–9, 60 °C, 3.92 mg/mL, 0.27 U/mL), respectively. The protease activities of FI and FII could be activated by the surfactant SDS (1%). The fifth day culture supernatant enhanced the growth of Chinese cabbage (Brassica campestris). The enhancing effect was due to hydrolyzates of the metabolites rather than the proteases.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Enzyme and Microbial Technology - Volume 40, Issue 5, 3 April 2007, Pages 1213–1220
نویسندگان
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