کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
18254 42715 2007 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A composite enzymatic membrane for the specific separation and concentration of l glycerophosphate from a racemic d/l glycerophosphate mixture
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
A composite enzymatic membrane for the specific separation and concentration of l glycerophosphate from a racemic d/l glycerophosphate mixture
چکیده انگلیسی

A composite enzymatic membrane (CEM), made up of two enzymatic membranes carrying two enzymes catalyzing two opposite reactions on either side of a permeable negatively charged membrane was used in this study. The phosphatase/kinase enzyme couple was chosen to make CEM permitting the active and selective transport of small-phosphorylated molecules. Here, we show that a CEM involving the alkaline phosphatase/glycerokinase enzyme couple permits, in a compartmentalized system, not only the separation of the d and l forms of a racemic mixture of (d/l) glycerophosphate, but also the simultaneous concentration of the l form alone.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Enzyme and Microbial Technology - Volume 40, Issue 6, 2 May 2007, Pages 1604–1607
نویسندگان
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