کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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182889 | 459527 | 2016 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Oxidation of the Flavonolignan Silybin. In situ EPR Evidence of the Spin-Trapped Silybin Radical Oxidation of the Flavonolignan Silybin. In situ EPR Evidence of the Spin-Trapped Silybin Radical](/preview/png/182889.png)
• The specific new redox behavior of bioactive molecule of silybin is presented.
• The electrochemically generated silybin radical was detected for the first time.
• BMPO spin-trap was successfully applied by in situ EPR spectroelectrochemistry.
• The electrochemical oxidation mechanism of silybin is elucidated.
Oxidation of natural flavonolignan silybin in an aprotic environment was studied by electrochemical methods, electron paramagnetic resonance (EPR), UV–vis and IR in situ spectroelectrochemistry and chromatographic techniques. The presence of electrochemically generated silybin radical was proven using in situ EPR spectroelectrochemistry by spin trapping method with 5-tert-butoxycarbonyl-5-methyl-1-pyrroline-N-oxide (BMPO) as a spin trap reagent. The adduct of the silybin radical with BMPO was additionally unambiguously identified by UPLC-ESI-MS/MS. For the first time, the flavonolignan radical was detected upon electrochemical oxidation under such conditions. The one-electron oxidation of silybin leads to a hydroxylated silybin derivative. Its specific redox behaviour can reflect its bioefficiency.
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Journal: Electrochimica Acta - Volume 205, 1 July 2016, Pages 118–123