کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
18368 | 42720 | 2007 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Amperometric immunosensor for the detection of anti-West Nile virus IgG using a photoactive copolymer
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موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
A polymeric film consisting of two distinct monomers such as pyrrole–benzophenone and tris(bipyridine pyrrole) ruthenium (II) units was electrogenerated and used for the photochemical grafting of T7 bacteriophages displaying a specific West Nile virus (WNV) epitope. The resulting electrodes were applied to the amperometric detection of WNV antibody at 0 V in aqueous electrolyte via secondary peroxidase-labeled antibody, hydrogen peroxide and hydroquinone as the enzyme substrate. A calibration curve based on different WNV–antibody dilutions ranging from 10 to 106 was created with a relatively short response time of 30 s after substrate injection.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Enzyme and Microbial Technology - Volume 40, Issue 3, 5 February 2007, Pages 403–408
Journal: Enzyme and Microbial Technology - Volume 40, Issue 3, 5 February 2007, Pages 403–408
نویسندگان
Rodica E. Ionescu, Serge Cosnier, Gregoire Herzog, Karine Gorgy, Boaz Leshem, Sebastien Herrmann, Robert S. Marks,