کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
18396 42721 2006 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Production of isoamyl acetate with immobilized Staphylococcus simulans lipase in a solvent-free system
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Production of isoamyl acetate with immobilized Staphylococcus simulans lipase in a solvent-free system
چکیده انگلیسی

A non-commercial immobilized lipase preparation from Staphylococcus simulans was used to catalyze the esterification reaction between acetic acid and isoamyl alcohol to synthetize isoamyl acetate (banana flavour) in pure substrate conditions (without addition of any organic solvent). The effects of various reaction parameters such as the amount of lipase, the molar ratio of acetic acid as compared to isoamyl alcohol and the amount of initially added water were investigated. The optimum conditions for achieving 64% conversion during 8 h of incubation are 60 IU of immobilized lipase, an acid/alcohol of 2 molar ratio and an initial addition of water of 10% (w/w). No significant loss of the immobilized lipase activity was observed after four cycles of use of the immobilized enzymatic preparation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Enzyme and Microbial Technology - Volume 38, Issue 6, 1 April 2006, Pages 788–794
نویسندگان
, , , , ,