کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
18485 42725 2006 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Enzymatic hydrolysis of phospholipids from Isochrysis galbana for docosahexaenoic acid enrichment
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Enzymatic hydrolysis of phospholipids from Isochrysis galbana for docosahexaenoic acid enrichment
چکیده انگلیسی

This paper describes the use of lipases for the production of a lipid fraction rich in docosahexaenoic acid (DHA) using phospholipids from the microalga Isochrysis galbana as raw material.Fatty acid compositions of total lipids and lipid classes of I. galbana were studied. DHA accounted for 13.5% of total fatty acids. However, the DHA content of the phospholipid fraction was 50% and 75% of total DHA was carried by phospholipids, mainly at the sn-2 position. These phospholipids contained neither eicosapentaenoic acid nor arachidonic acid.Selective phospholipid hydrolysis using 12 lipase preparations has been studied in order to produce lipid fractions enriched in DHA. Five lipases showed discrimination between DHA and other fatty acids.A lipid fraction containing 80% of DHA was obtained with two lipases. Even though both preparations were equivalent in terms of DHA enrichment (1.5–1.6), lipase from Rhizopus oryzae gave better results as far as DHA recovery (85%) is concerned. Lipase from Mucor javanicus gave a slightly lower recovery yield (71%) for the same reaction time (3 h).

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Enzyme and Microbial Technology - Volume 39, Issue 4, 2 August 2006, Pages 548–554
نویسندگان
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