Preparation and assembly of collagen–DNA complex on an electrode surface and its application to protein analysis

• The complex of collagen and DNA has been prepared on the electrode surface.
• The prepared tubular complex was developed to be a new platform for protein analysis.
• A simple but sensitive electrochemical biosensor for assays of MMP-2 was developed.
• Entrapment of target proteins onto the electrode was achieved.

In this work, we have prepared collagen–DNA complex on the surface of an electrode through electrostatic interaction and hydrogen bonding between collagen triple helix and DNA double helix. The prepared tubular material is then deposited on the electrode surface to form a film. Due to the excellent biocompatibility of collagen and DNA, the prepared biomaterial can provide a new platform for protein analysis. On the one hand, since collagen in the complex is the substrate of matrix metalloproteinases-2 (MMP-2), a new kind of electrochemical biosensor for the detection of MMP-2 can be developed. On the other, the collagen–DNA complex can be used to entrap proteins onto the electrode surface with the activity of the target proteins being well maintained, thus further analysis can be conducted on the target proteins.

Figure optionsDownload as PowerPoint slide