کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
18746 42741 2006 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Inhibition of Clostridium histolyticum supernatant cytotoxic activity by protease inhibitors
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Inhibition of Clostridium histolyticum supernatant cytotoxic activity by protease inhibitors
چکیده انگلیسی

Clostridium histolyticum supernatant, thanks to intrinsic proteases, exerts cytotoxic effect on cells and tissues. In our study we compared the ability of five different protease inhibitors to block cytotoxic activity of C. histolyticum supertanant: aprotinin, leupeptin, phenylmethylsulphonyl fluoride (PMSF), l-1-chloro-3-[4-tosylamido]-7-amino-2-heptanone-HCl (TLCK) and chymostatin. Inhibitors of serine proteases nearly completely blocked cytotoxic effect, especially in lower supernatant concentrations. At the same time TLCK, known to irreversibly block clostripain obtained from C. histolyticum, blocked enzymatic activity of clostripain, but not cytotoxic effect of the supernatant. We conclude that the remaining cytotoxicity must be the effect of the activity of another factor that is contained in the supernatant and that can be isolated and tested in the presence of TLCK.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Enzyme and Microbial Technology - Volume 39, Issue 1, 1 June 2006, Pages 28–31
نویسندگان
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