کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1877338 | 1042070 | 2006 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Measuring the affinity of a radioligand with its receptor using a rotating cell dish with in situ reference area
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
فیزیک و نجوم
تشعشع
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Measuring the affinity of a radioligand with its receptor using a rotating cell dish with in situ reference area Measuring the affinity of a radioligand with its receptor using a rotating cell dish with in situ reference area](/preview/png/1877338.png)
چکیده انگلیسی
This report describes a semi-automated method for the measurement of affinity of radiolabeled ligands interacting with cell-surface receptors on intact cancer cells. The method saves labor time and reagents compared to common manual measurements. A complete affinity measurement can be performed in one cell dish by using a target cell area and a reference area and repeatedly measure the differential activity (i.e. target activity—reference activity). The affinities obtained for different ligand–receptor interactions agreed with affinities reported in the literature.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Applied Radiation and Isotopes - Volume 64, Issue 1, January 2006, Pages 32–37
Journal: Applied Radiation and Isotopes - Volume 64, Issue 1, January 2006, Pages 32–37
نویسندگان
Henrik Björke, Karl Andersson,