کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1882646 | 1533543 | 2011 | 6 صفحه PDF | دانلود رایگان |

The aim of the present work is to study the effect of gamma radiation on the lipid part of the erythrocyte membrane, and to test the efficiency of lipoic acid as a radioprotector. This effect was evaluated using electron paramagnetic resonance (EPR), and Fourier transform infrared (FT-IR) spectroscopy. The results showed an increase in the number of spin density by 14%, 22% and 65% after exposure to 25, 50 and 100 Gy respectively; whereas there was a decline in the obtained density after incubation with lipoic acid by a factor of approximately 32%. The FT-IR spectra of the irradiated erythrocytes samples showed a marked decrease in the intensity of all characteristic peaks, which increased as the irradiation dose increased. The second-derivative of these spectra, allow the conformationally sensitive membrane acyl chain methylene stretching modes to be separated from the protein (mostly hemoglobin) vibrations that dominate the spectra of intact cells. The 2850 cm−1 band showed changes in the band shape and position after exposure to 50 and 100 Gy. Therefore it can be concluded that the band at 2850 cm−1 only is useful in monitoring the radiation effect of the lipids cell membrane intact cells.
► Effect of γ radiation on erythrocyte membrane was studied using EPR and FT-IR.
► Efficiency of α-lipoic acid as radioprotector was tested.
► Lipoic acid diminished the free radicals number after gamma irradiation by 32%.
► FT-IR spectra of the irradiated erythrocyte showed a decrease in their intensity.
► Lipoic acid enhances the membrane to resist the action of gamma radiation.
Journal: Radiation Physics and Chemistry - Volume 80, Issue 12, December 2011, Pages 1337–1342