کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1883084 1043277 2014 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Accuracy of 2-hydroxyglutarate quantification by short-echo proton-MRS at 3 T: A phantom study
موضوعات مرتبط
مهندسی و علوم پایه فیزیک و نجوم تشعشع
پیش نمایش صفحه اول مقاله
Accuracy of 2-hydroxyglutarate quantification by short-echo proton-MRS at 3 T: A phantom study
چکیده انگلیسی

PurposeWe set out to investigate the potential confounding effect of variable concentration of N-acetyl-l-aspartate (NAA) and Glutamate (Glu) on measurement of the brain oncometabolite 2-hydroxyglutarate (2HG) using a standard MRS protocol. This issue may arise due to spectral overlap at clinical magnetic field strengths and thus complicate the usage of 2HG as a putative biomarker of gliomas bearing mutations of the isocitrate dehydrogenase (IDH) 1 and 2 genes.MethodsSpectra from 25 phantoms (50 mL falcon test tubes) containing a range of known concentrations of 2HG, NAA and Glu were acquired using a clinical 3 T scanner with a quadrature head coil, single-voxel point-resolved spectroscopy sequence with TE = 30 ms. Metabolite concentrations were estimated by linear combination analysis and a simulated basis set.ResultsNAA and Glu concentrations can have a significant confounding effect on 2HG measurements, whereby the negative changes in concentration of these metabolites typically observed in (peri)lesional areas can lead to under-estimation of 2HG concentration with respect to spectra acquired in presence of physiological levels of NAA and Glu.ConclusionThe confounding effect of NAA and Glu concentration changes needs to be considered: in patients, it may mask the presence of 2HG at low concentrations, however it is not expected to lead to false positives. 2HG data acquired using standard short echo-time MRS protocols should be considered with caution.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Physica Medica - Volume 30, Issue 6, September 2014, Pages 702–707
نویسندگان
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