کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
189503 459680 2011 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Generation of a doxorubicin immunosensor based on a specific monoclonal antibody-nanogold-modified electrode
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی مهندسی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
Generation of a doxorubicin immunosensor based on a specific monoclonal antibody-nanogold-modified electrode
چکیده انگلیسی

A highly sensitive immunosensor based on immobilization of specific monoclonal antibodies onto gold nanoparticles (GNPs) attached to a modified gold electrode was developed for the determination of the presence of doxorubicin. Colloidal GNPs were coated on a gold electrode using the thiol groups of 1,6-hexanedithiol (HDT) as a cross-linker. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were employed for characterization of the various layers that were coated onto the electrode. The relative charge transfer resistance of the antibody-modified gold electrode changed linearly with the concentration of doxorubicin. The limit of detection (3 sb/m) was 0.83 pg mL−1, and a linear range was obtained from 1.0 to 160.0 pg mL−1 of doxorubicin. The affinity of doxorubicin for the immobilized antibody was determined to be 1.74 × 1011 M−1. The sensitivity and specificity of the immunosensor was validated using doxorubicin-spiked human serum samples, and the results show that the immunosensor is a useful tool for screening picogram amounts of doxorubicin in the clinical laboratory.


► A highly sensitive immunosensor is proposed for doxorubicin.
► A specific monoclonal antibody is applied as a selective electrode sites.
► As a modifier, GNPs was used for sensitive determination.
► Doxorubicin-spiked human serum sample was successfully determined.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Electrochimica Acta - Volume 56, Issue 16, 30 June 2011, Pages 5702–5706
نویسندگان
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