Wiring horseradish peroxidase on gold nanoparticles-based nanostructured polymeric network for the construction of mediatorless hydrogen peroxide biosensor

Gold electrodes were functionalized with an electropolymerized matrix of Au nanoparticles modified with 2-mercaptoethanesulfonic acid, 3-mercaptophenyl boronic acid and p-aminothiophenol. The resulting nanostructured electroconductive matrix was used as support for the oriented immobilization of horseradish peroxidase to construct a reagentless amperometric biosensor for H2O2. The electrode, poised at 0.0 mV, exhibited a rapid response within 8 s and a linear calibration range from 5 μM to 1.1 mM H2O2. The sensitivity of the biosensor was determined as 498 μA/M cm2, and its detection limit was 1.5 μM H2O2 at a signal-to-noise ratio of 3. The electrode retained 95% and 72% of its initial activity after 21 and 40 days of storage at 4 °C.

Figure optionsDownload as PowerPoint slideHighlights
► Gold substrates modified with a conductive polymer of aniline and boronic acid AuNPs were developed.
► They were used for the oriented immobilization of the glycoenzyme HRP.
► The approach was applied for the construction of a reagentless biosensor for H2O2.
► It exhibits good sensitivity, stability and fast electroanalytical response.