کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1908679 1046679 2012 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A ratiometric fluorescent probe for assessing mitochondrial phospholipid peroxidation within living cells
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی سالمندی
پیش نمایش صفحه اول مقاله
A ratiometric fluorescent probe for assessing mitochondrial phospholipid peroxidation within living cells
چکیده انگلیسی

Mitochondrial oxidative damage contributes to a wide range of pathologies, and lipid peroxidation of the mitochondrial inner membrane is a major component of this disruption. However, despite its importance, there are no methods to assess mitochondrial lipid peroxidation within cells specifically. To address this unmet need we have developed a ratiometric, fluorescent, mitochondria-targeted lipid peroxidation probe, MitoPerOx. This compound is derived from the C11-BODIPY581/591 probe, which contains a boron dipyromethane difluoride (BODIPY) fluorophore conjugated via a dienyl link to a phenyl group. In response to lipid peroxidation the fluorescence emission maximum shifts from ∼590 to ∼520 nm. To target this probe to the matrix-facing surface of the mitochondrial inner membrane we attached a triphenylphosphonium lipophilic cation, which leads to its selective uptake into mitochondria in cells, driven by the mitochondrial membrane potential. Here we report on the development and characterization of MitoPerOx. We found that MitoPerOx was taken up very rapidly into mitochondria within cells, where it responded to changes in mitochondrial lipid peroxidation that could be measured by fluorimetry, confocal microscopy, and epifluorescence live cell imaging. Importantly, the peroxidation-sensitive change in fluorescence at 520 nm relative to that at 590 nm enabled the use of the probe as a ratiometric fluorescent probe, greatly facilitating assessment of mitochondrial lipid peroxidation in cells.

Figure optionsDownload high-quality image (78 K)Download as PowerPoint slideHighlights
► A mitochondria-targeted lipid peroxidation probe is described.
► The probe, MitoPerOx, is taken up rapidly by mitochondria in cells.
► Changes in MitoPerOx fluorescence enable the assessment of mitochondrial lipid peroxidation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 53, Issue 3, 1 August 2012, Pages 544–553
نویسندگان
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