کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1908823 | 1534989 | 2012 | 8 صفحه PDF | دانلود رایگان |
In this paper, using the monocyte/macrophage cell line RAW264.7, we systematically investigate the impact of macrophage enrichment with unsaturated fatty acids on cellular radical synthesis. We found that the intracellular production of reactive nitrogen and oxygen intermediates depends on the activation status of the macrophages. For unstimulated macrophages PUFA enrichment resulted in an increase in cellular radical synthesis. For stimulated macrophages, instead, an impeding action of unsaturated fatty acids on the respiratory burst could be seen. Of particular importance, the impact of unsaturated fatty acids on the macrophage respiratory burst was also observed in RAW264.7 cells cocultivated with viable bacteria of the species Rhodococcus equi or Pseudomonas aeruginosa. PUFA supplementation of macrophages in the presence of R. equi or P. aeruginosa reduced the pathogen-stimulated synthesis of reactive nitrogen and oxygen intermediates. Furthermore, the unsaturated fatty acids were found to impede the expression of the myeloperoxidase gene and to reduce the activity of the enzyme. Hence, our data provide indications of a possible value of PUFA application to people suffering from chronic infections with R. equi and P. aeruginosa as a concomitant treatment to attenuate an excessive respiratory burst.
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► Membrane lipid composition interrelates with macrophage (MΦ) respiratory burst.
► Unstimulated MΦ shows an increase in reactive nitrogen and oxygen intermediate synthesis after PUFA supplementation.
► In activated MΦ PUFA has an impeding action on stimulator-induced respiratory burst.
► The PUFA effects are evidenced in macrophages cocultivated with viable bacteria.
Journal: Free Radical Biology and Medicine - Volume 52, Issues 11–12, 1–15 June 2012, Pages 2246–2253