کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1909719 1046738 2009 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Measuring the poise of thiol/disulfide couples in vivo
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی سالمندی
پیش نمایش صفحه اول مقاله
Measuring the poise of thiol/disulfide couples in vivo
چکیده انگلیسی

The reduction potentials (Eh) for the redox couples GSH/GSSG and cysteine/cystine (Cys/CySS) in plasma are useful indicators of systemic oxidative stress and other medically relevant physiological states. This article describes a sensitive method for determining plasma levels of GSH, GSSG, Cys, and CySS used to calculate the in vivo Eh values. The method uses iodoacetate to alkylate free thiols, derivatization with dansyl chloride to fluorescently tag amino groups, and HPLC and fluorescence to separate, detect, and quantify the molecules. Benefits of the method, such as sensitivity and dynamic range, are described, as are caveats, such as the importance of preventing red blood cell hemolysis and limitations in quantification of GSSG. General principles of redox chemistry and previous studies showing that the compounds are more oxidized than predicted from their standard reduction potentials are reviewed. The calculated in vivo Eh is a convenient and informative way of summarizing the redox environment of plasma and is also useful for studies of cerebrospinal fluid, lymph, bronchoalveolar lavage fluid, human biopsies, and a broad range of in vitro cell culture conditions.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 47, Issue 10, 15 November 2009, Pages 1329–1338
نویسندگان
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