کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1910850 | 1046789 | 2008 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Degradation of extracellular matrix by peroxynitrite/peroxynitrous acid
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
1,9-dimethylmethylene blueVascular smooth muscle cells.PBSDMPODMEMDMMBSIN-1VSMCECM3-morpholinosydnonimine - 3-مورفولینویدونیمینیم5,5-dimethyl-1-pyrroline N-oxide - 5،5-دی متیل-1-پیرولین N-اکسیدDulbecco's modified Eagle's medium - Medal of Eagle اصلاح شده DulbeccoAtherosclerosis - آترواسکلروز(تصلب شریان)Protein oxidation - اکسیداسیون پروتئینEnzyme-linked immunosorbent assay - تست الیزاELISA - تست الیزاFree radicals - رادیکال آزادExtracellular matrix - ماتریکس خارج سلولیPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریnitrotyrosine - نیتروتیروستینNitric oxide - نیتریک اکسیدPeroxynitrite - پروکسی نیتریتGlycosaminoglycan - گلیکوزآمینوگلیکانImmuno-spin trapping - گیر کردن اسپین ایمن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The extracellular matrix (ECM) provides strength and elasticity to tissues and plays a key role in regulating cell behavior; damage to this material is believed to be a major factor in many inflammatory diseases. Peroxynitrite/peroxynitrous acid, which is generated at elevated levels at sites of inflammation, is believed to play a role in ECM damage; however, the mechanisms involved are poorly understood. Here we examined the reactions of bolus peroxynitrite, and that generated in a time-dependent manner by SIN-1 decomposition, with ECM isolated from a vascular smooth muscle cell line and porcine thoracic aorta. Bolus peroxynitrite caused the release of ECM glycosaminoglycans and proteins, the formation of 3-nitroTyr, and the detection of ECM-derived radicals (by immuno-spin trapping) in a concentration-dependent manner. Release and nitration of ECM components were modulated by the local pH and bicarbonate. SIN-1 caused the release of glycosaminoglycan, but not protein, from vascular smooth muscle cell-derived ECM in a concentration-, time-, and pH-dependent manner. The data presented here suggest that peroxynitrite-mediated damage to ECM occurs via a radical-mediated pathway. These reactions may contribute to ECM damage at sites of inflammation and play a role in disease progression, including rupture of atherosclerotic lesions.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 45, Issue 5, 1 September 2008, Pages 716-725
Journal: Free Radical Biology and Medicine - Volume 45, Issue 5, 1 September 2008, Pages 716-725
نویسندگان
Eleanor C. Kennett, Michael J. Davies,