کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1911147 | 1046803 | 2006 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Redox modulation of the hepatitis C virus replication complex is calcium dependent
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کلمات کلیدی
FBSDMEM1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid tetrakis acetoxymethyl esterBSOIFN-γEGTAIP3GSHGAPDHDMSO - DMSODulbecco's modified Eagle Medium - Eagle Medium اصلاح شده DulbeccoInositol 1,4,5-triphosphate - Inositol 1،4،5-تری فسفات[Ca2+]i - [Ca2 +] iGlucose oxidase - آنزیم گلوکزاکسیدازinterferon-γ - اینترفرون-γBAPTA-AM - بیایپیتیای-AMDimethyl sulfoxide - دیمتیل سولفواکسیدfetal bovine serum - سرم جنین گاوendoplasmic reticulum - شبکه آندوپلاسمی intracellular calcium concentration - غلظت کلسیم داخل سلولیHepatitis C virus - هپاتیت سیHCV - هپاتیت سیGlutathione - گلوتاتیونglyceraldehyde-3-phosphate dehydrogenase - گلیسرالیدید-3-فسفات دهیدروژناز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Reactive species and perturbation of the redox balance have been implicated in the pathogenesis of many viral diseases, including hepatitis C. Previously, we made a surprising discovery that concentrations of H2O2 that are nontoxic to host cells disrupted the hepatitis C virus (HCV) replication complex (RC) in Huh7 human hepatoma cells in a manner that suggested signaling. Here, we show that H2O2 and interferon-γ have comparable effects on the HCV subgenomic and genomic RNA replication in Huh7 cells. H2O2 induced a gradual rise in the intracellular calcium concentration ([Ca2+]i). Both rapid and sustained suppression of HCV RNA replication by H2O2 depended on this calcium elevation. The peroxide-induced [Ca2+]i elevation was independent of extracellular calcium and derived, at least in part, from the endoplasmic reticulum. Likewise, the suppression of the HCV RC by H2O2 was independent of extracellular calcium but required an intracellular calcium source. Other agents that elevated [Ca2+]i could also suppress the HCV RC, suggesting that calcium elevation might be sufficient to suppress HCV RNA replication. In conclusion, oxidants may modulate the HCV RC through calcium. Effects on the infectivity and the morphogenesis of HCV remain to be determined. These findings suggest possible regulatory roles for redox and calcium signaling during viral infections.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 41, Issue 9, 1 November 2006, Pages 1488-1498
Journal: Free Radical Biology and Medicine - Volume 41, Issue 9, 1 November 2006, Pages 1488-1498
نویسندگان
Jinah Choi, Henry Jay Forman, Jing-hsiung Ou, Michael M.C. Lai, Scott Seronello, Anna Nandipati,