کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1911835 | 1046837 | 2006 | 9 صفحه PDF | دانلود رایگان |
The oncoprotein c-Myc is essential for thymocyte development, and its dysregulation causes lymphoid malignancies. We have demonstrated previously that spontaneous DNA synthesis in Atm−/− thymocytes is markedly increased over that of Atm+/+ thymocytes and that glucocorticoid dexamethasone suppresses thymocyte DNA synthesis and prevents the ultimate development of thymic lymphoma in Atm−/− mice. Recently, we reported that in Atm−/− thymic lymphoma cells c-Myc is overexpressed compared with the levels of c-Myc in primary thymocytes from wild-type or Atm−/− mice. In this study, we show that c-Myc expression progressively increases with age in primary thymocytes from Atm−/− mice and that the upregulation of c-Myc parallels the elevated DNA synthesis in the cells, suggesting that deregulation of c-Myc may drive the uncontrolled proliferation of thymocytes in Atm−/− mice. Here we also demonstrate that Atm−/− thymocytes exhibit increased levels of hydrogen peroxide, NF-E2-related factor (Nrf-2), peroxiredoxin-1, and intracellular glutathione relative to thymocytes from Atm+/+ mice. Importantly, reduction of hydrogen peroxide by administration of the antioxidant N-acetylcysteine to Atm−/− mice attenuates the elevation of Nrf-2, c-Myc, and DNA synthesis in their thymocytes, suggesting that ATM may control c-Myc and DNA synthesis during postnatal thymocyte development by preventing accumulation of reactive oxygen species.
Journal: Free Radical Biology and Medicine - Volume 41, Issue 4, 15 August 2006, Pages 640–648