کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1911965 | 1046852 | 2006 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Comparison of stable nitroxide, 3-substituted 2,2,5,5-tetramethylpyrrolidine-N-oxyls, with respect to protection from radiation, prevention of DNA damage, and distribution in mice
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کلمات کلیدی
FBS4-hydroxy-2,2,6,6-tetramethylpiperidine-N-oxylsupercoiledIn vivo distribution8-OHdG8-hydroxydeoxyguanosine - 8 هیدروکسی دیواکسی گوآزینintraperitoneal(ly) - intraperitoneal (ly)DNA cleavage - تجزیه DNAopen circular - دایره بازFree radical - رادیکالهای آزاد Colony Formation - سازند کلنیSOD - سدfetal bovine serum - سرم جنین گاوTempol - سریعSuperoxide dismutase - سوکسوکس دیسموتازbody weight - وزن بدنRedox potential - پتانسیل RedoxPROXYL - پروکسیل
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Comparison of stable nitroxide, 3-substituted 2,2,5,5-tetramethylpyrrolidine-N-oxyls, with respect to protection from radiation, prevention of DNA damage, and distribution in mice Comparison of stable nitroxide, 3-substituted 2,2,5,5-tetramethylpyrrolidine-N-oxyls, with respect to protection from radiation, prevention of DNA damage, and distribution in mice](/preview/png/1911965.png)
چکیده انگلیسی
We compared three 3-substituted 2,2,5,5-tetramethylpyrrolidine-N-oxyls (PROXYLs): carbamoyl-, methoxycarbonyl-, and hydroxymethyl-PROXYL (CM-, MC-, and HM-PROXYL, respectively) with respect to radioprotection, prevention of DNA damage, and in vivo distribution in mice. The PROXYLs provided protection to C3H mice against lethal X-irradiation (8 Gy) with the following order of magnitude, HM- > CM- â MC-PROXYL. In contrast, radioprotection at the cellular level assessed by the colony formation of leukemia cell line L5178Y showed no difference among them. The degree of protection from X ray-induced oxidation of DNA bases measured by the formation of 8-hydroxydeoxyguanosine in salmon DNA and the cleavage of DNA measured by electrophoresis of plasmid pBR322 DNA did not differ among the PROXYLs. Redox potentials were also similar for each. However, the blood concentration of the PROXYLs injected ip into the mice showed different maximum concentrations (HM- > CM- â MC-PROXYL), although all reached a maximum at around 5-10 min and gradually decreased thereafter. Their concentration in bone marrow showed a similar pattern, suggesting that the difference in in vivo radioprotection among the three PROXYLs is due to the difference in their distribution to bone marrow. In general, the radioprotection provided by stable nitroxides is affected not only by redox potential and reactivity in vitro but also by pharmacokinetics.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 40, Issue 7, 1 April 2006, Pages 1170-1178
Journal: Free Radical Biology and Medicine - Volume 40, Issue 7, 1 April 2006, Pages 1170-1178
نویسندگان
Kazunori Anzai, Megumi Ueno, Akira Yoshida, Masako Furuse, Winn Aung, Ikuo Nakanishi, Takashi Moritake, Keizo Takeshita, Nobuo Ikota,