کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
191612 | 459725 | 2010 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Direct electrochemistry and electrocatalysis of heme-proteins immobilized in porous carbon nanofiber/room-temperature ionic liquid composite film Direct electrochemistry and electrocatalysis of heme-proteins immobilized in porous carbon nanofiber/room-temperature ionic liquid composite film](/preview/png/191612.png)
The combination of porous carbon nanofiber (PCNF) and room-temperature ionic liquid (RTIL) provided a suitable microenvironment for heme-proteins to transfer electron directly. Hemoglobin, myoglobin, and cytochrome c incorporated in PCNF/RTIL films exhibited a pair of well-defined, quasi-reversible cyclic voltammetric peaks at about −0.28 V vs. SCE in pH 7.0 buffers, respectively, characteristic of the protein heme Fe(III)/Fe(II) redox couples. The cyclic voltammetry and electrochemical impedance spectroscopy were used to characterize the modified electrode. The heme/PCNF/RTIL/CHIT films were also characterized by UV–vis spectroscopy, indicating that heme-proteins in the composite film could retain its native structure. Oxygen, hydrogen peroxide, and nitrite were catalytically reduced at the heme/PCNF/RTIL/CHIT film modified electrodes, showing the potential applicability of the films as the new type of biosensors or bioreactors based on direct electrochemistry of the redox proteins.
Journal: Electrochimica Acta - Volume 55, Issue 9, 30 March 2010, Pages 3185–3191