کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1925059 | 1536337 | 2015 | 8 صفحه PDF | دانلود رایگان |
• HSP20 suppressed the growth of HCC cells in the presence of TNF-α.
• HSP20 decreased the IKK-α expression in HCC cells.
• HSP20 suppressed the TNF-α-stimulated NF-κB signaling in HCC cells.
• The level of IKK-α was inversely proportional to that of HSP20 in human HCC tissue specimens.
• HSP20 down-regulates the TNF-α-stimulated NF-κB signaling in HCC.
We previously demonstrated that the expression of HSP20, a small heat shock protein, is inversely correlated with the progression of HCC. Inflammation is associated with HCC, and numerous cytokines, including TNF-α, act as key mediators in the progression of HCC. In the present study, we investigated whether HSP20 is implicated in the TNF-α-stimulated intracellular signaling in HCC using human HCC-derived HuH7 cells in the presence of TNF-α. In HSP20-overexpressing HCC cells, the cell growth was retarded compared with that in the control cells under long-term exposure of TNF-α. Because NF-κB pathway is the main intracellular signaling system activated by TNF-α, we investigated the effects of HSP20-overexpression of this pathway. The protein levels of IKK-α, but not IKK-β, in the HSP20-overexpressing cells were decreased. Short-term exposure to TNF-α-induced phosphorylation and degradation of IκB, and the phosphorylation and transactivational activity of NF-κB were suppressed in the HSP20-overexpressing HCC cells. Furthermore, the increase in IKK-α levels was accompanied by a decrease in the HSP20 levels in human HCC tissues. These findings strongly suggest that HSP20 might decrease the IKK-α protein level and that it down-regulates the TNF-α-stimulated intracellular signaling in HCC, thus resulting in the suppression of HCC progression.
Journal: Archives of Biochemistry and Biophysics - Volume 565, 1 January 2015, Pages 1–8