کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1926210 | 1536441 | 2010 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Decolorization of malachite green by cytochrome c in the mitochondria of the fungus Cunninghamella elegans Decolorization of malachite green by cytochrome c in the mitochondria of the fungus Cunninghamella elegans](/preview/png/1926210.png)
We studied the decolorization of malachite green (MG) by the fungus Cunninghamella elegans. The mitochondrial activity for MG reduction was increased with a simultaneous increase of a 9-kDa protein, called CeCyt. The presence of cytochrome c in CeCyt protein was determined by optical absorbance spectroscopy with an extinction coefficient (E550–535) of 19.7 ± 6.3 mM−1 cm−1 and reduction potential of + 261 mV. When purified CeCyt was added into the mitochondria, the specific activity of CeCyt reached 440 ± 122 μmol min−1 mg−1 protein. The inhibition of MG reduction by stigmatellin, but not by antimycin A, indicated a possible linkage of CeCyt activity to the Qo site of the bc1 complex. The RT-PCR results showed tight regulation of the cecyt gene expression by reactive oxygen species. We suggest that CeCyt acts as a protein reductant for MG under oxidative stress in a stationary or secondary growth stage of this fungus.
Journal: Archives of Biochemistry and Biophysics - Volume 494, Issue 2, 15 February 2010, Pages 159–165