کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1926459 | 1536464 | 2009 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: E-cadherin decreased human breast cancer cells sensitivity to staurosporine by up-regulating Bcl-2 expression E-cadherin decreased human breast cancer cells sensitivity to staurosporine by up-regulating Bcl-2 expression](/preview/png/1926459.png)
E-cadherin, a well-characterized cell–cell adhesion molecule, executes multifunction roles on cell behaviors. However, its effect on chemo-resistance remains controversial. In this study, we found that E-cadherin positive breast cell lines were less sensitive to staurosporine compared to E-cadherin negative ones. Next, we substantiated that the expression of E-cadherin in MDA-MB-435 cells could partly counteract the cytotoxic effect induced by staurosporine through a series of apoptosis assay. The resistance of E-cadherin over-expressing cells to staurosporine may due to the up-regulation of Bcl-2/Bax ratio. When E-cadherin interference plasmids were transfected into MCF-7 cells, Bcl-2 expression was down-regulated. Moreover, perturbation of E-cadherin function by blocking the cell–cell contact resulted in decreased cellular levels of Bcl-2 protein expression. All these results demonstrated the chemo-resistance function of E-cadherin in the condition of staurosporine treatment, therefore, might contribute effective therapeutic strategies in breast carcinoma.
Journal: Archives of Biochemistry and Biophysics - Volume 481, Issue 1, 1 January 2009, Pages 116–122