کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1926910 1536487 2008 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Mono-O-methylated flavanols and other flavonoids as inhibitors of endothelial NADPH oxidase
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Mono-O-methylated flavanols and other flavonoids as inhibitors of endothelial NADPH oxidase
چکیده انگلیسی

The dietary flavan-3-ol (−)-epicatechin improves the bioactivity of nitric oxide in arterial vessels in vivo. Moreover, it effectively protects cultured vascular endothelial cells from signs of oxidative stress and elevates intracellular nitric oxide in vitro. We addressed the effects of (−)-epicatechin, its metabolic conversion products and structurally related compounds on NADPH oxidase activity in intact human umbilical vein endothelial cells (HUVEC) and in cell lysates. (−)-Epicatechin proved to be an O2−-scavenger but did not inhibit NADPH oxidase activity, whereas the converse pattern was observed for the metabolites 3′- and 4′-O-methyl epicatechin. The dimer procyanidin B2 and (−)-epicatechin glucuronide were O2−-scavengers and inhibited NADPH oxidase. Analysis of structure–activity relations with 45 compounds suggests an apocynin-like mode of NADPH oxidase inhibition. Notably, HUVEC converted (−)-epicatechin to NADPH oxidase-inhibitory methyl ethers. These data identify endothelial NADPH oxidase as candidate target of dietary flavonoids and particularly of their metabolites.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Archives of Biochemistry and Biophysics - Volume 469, Issue 2, 15 January 2008, Pages 209–219
نویسندگان
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