کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1927242 | 1536506 | 2007 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Regulation of protein phosphorylation within the MKK1-ERK2 complex by MP1 and the MP1â¢P14 heterodimer
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
MEK partner 1 (MP1) and P14 are small proteins that modulate the Raf-MKK1/2-ERK1/2 pathway. To examine the biochemical basis for their function a fluorescent form of MP1 was prepared by labeling Cys-74 with fluorescein. Using this protein it was shown that MP1 binds unactivated ERK1, ERK2 and a constitutively active form of MKK1 (MKK1G7B) with dissociation constants of 9.7 ± 1.6, 3.3 ± 0.6 and 2.2 ± 0.5 μM, respectively. MP1 inhibits the ability of activated ERK2 to phosphorylate the transcription factor Ets-1. Both MP1 and the MP1
- P14 complex inhibit the ability of activated ERK2 to phosphorylate MKK1G7B, thus impeding feedback inhibition. In contrast, MP1 and the P14
- MP1 complex enhance the ability of MKK1G7B to phosphorylate ERK2, when ERK2 is present at a low concentration, but not when it is present at a high concentration. Thus, MP1 and the MP1
- P14 complex have the potential to differentially modulate activating and inhibiting signals in the Raf-MKK1/2-ERK1/2 pathway.
- P14 complex inhibit the ability of activated ERK2 to phosphorylate MKK1G7B, thus impeding feedback inhibition. In contrast, MP1 and the P14
- MP1 complex enhance the ability of MKK1G7B to phosphorylate ERK2, when ERK2 is present at a low concentration, but not when it is present at a high concentration. Thus, MP1 and the MP1
- P14 complex have the potential to differentially modulate activating and inhibiting signals in the Raf-MKK1/2-ERK1/2 pathway.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Archives of Biochemistry and Biophysics - Volume 460, Issue 1, 1 April 2007, Pages 85-91
Journal: Archives of Biochemistry and Biophysics - Volume 460, Issue 1, 1 April 2007, Pages 85-91
نویسندگان
Amrita Brahma, Kevin N. Dalby,