کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1927289 1536513 2006 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Localization of telokin at the intercalated discs of cardiac myocytes
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Localization of telokin at the intercalated discs of cardiac myocytes
چکیده انگلیسی
Telokin is identical in sequence to the C-terminal portion of myosin light chain kinase but is expressed independently. We have used monoclonal antibodies specific to the non-telokin portion of myosin light chain kinase and to telokin, immunofluorescence microscopy and image reconstruction to demonstrate the presence of telokin in cardiac myocytes and to study its subcellular distribution. Antibodies to telokin labeled the intercalated discs of adult cardiac myocytes and similar structures in isolated intercalated disc preparations. Antibodies specific to the non-telokin portion of myosin light chain kinase did not label intercalated discs in either of these preparations. Western blots of isolated intercalated discs with anti-telokin revealed a 23 kDa protein that co-migrates with purified telokin on SDS-PAGE. Deconvolution, reconstruction and analysis of fluorescence images of isolated intercalated discs labeled with anti-telokin and anti-β-catenin, anti-γ-catenin or anti-connexin43 indicated that telokin is only partially co-localized with these proteins at the discs.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Archives of Biochemistry and Biophysics - Volume 456, Issue 2, 15 December 2006, Pages 151-160
نویسندگان
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