کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1927632 | 1536532 | 2006 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Effect of five triterpenoid compounds isolated from root bark of Aralia elata on stimulus-induced superoxide generation, tyrosyl or serine/threonine phosphorylation and translocation of p47phox, p67phox, and rac to cell membrane in human neutrophils Effect of five triterpenoid compounds isolated from root bark of Aralia elata on stimulus-induced superoxide generation, tyrosyl or serine/threonine phosphorylation and translocation of p47phox, p67phox, and rac to cell membrane in human neutrophils](/preview/png/1927632.png)
Five triterpenoids; Congmuyanoside V, X, XI, XII, and XV (Cong. V, X, XI, XII, and XV) were isolated from the root bark of Aralia elata. The effect of these triterpenoids on stimulus-induced superoxide generation in human neutrophils was assayed by measuring the reduction of ferricytochrome c (cyt. c) using a dual-beam spectrophotometer. Translocation of p47phox, p67phox, and rac to the cell membrane and tyrosyl or serine/threonine phosphorylation of neutrophil proteins were investigated using specific monoclonal antibodies. The five triterpenoids used in the present experiment significantly suppressed N-formyl-methionyl-leucyl-phenylalanine (fMLP)-induced superoxide generation in concentration-dependent manner. Congmuyanoside V, XII, and XV also suppressed arachidonic acid (AA)-induced superoxide generation in high concentration. However, these triterpenoids showed no effect on phorbol 12-myristate 13-acetate (PMA)-induced superoxide generation. fMLP- and AA-induced tyrosyl phosphorylation and translocation of the cytosolic proteins p47phox, p67phox, and rac to the cell membrane were suppressed in parallel with the suppression of the stimulus-induced superoxide generation.
Journal: Archives of Biochemistry and Biophysics - Volume 446, Issue 1, 1 February 2006, Pages 84–90