کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1927830 | 1050262 | 2016 | 5 صفحه PDF | دانلود رایگان |
• GnIH and melatonin was significantly decreased during the sex change process.
• GnIH and melatonin receptor proteins were co-expressed in the hypothalamus.
• GnIH and melatonin plays an important role in the negative regulation of maturation during sex change.
The present study aimed to determine the relationship between melatonin and gonadotropin-inhibitory hormone (GnIH) and their effect on reproduction in cinnamon clownfish, Amphiprion melanopus. Accordingly, we investigated the expression pattern of GnIH, GnIH receptor (GnIH-R), and melatonin receptor (MT-R1) mRNA and protein, as well as the plasma levels of melatonin, during sex change in cinnamon clownfish. We found that GnIH and MT-R1 mRNA and melatonin activity were higher in fish with mature brain than in fish with developing gonads, and using double immunofluorescence staining, we found that both GnIH and MT-R1 proteins were co-expressed in the hypothalamus of cinnamon clownfish. These findings support the hypothesis that melatonin plays an important role in the negative regulation of maturation and GnIH regulation during reproduction.
Journal: Biochemical and Biophysical Research Communications - Volume 475, Issue 2, 24 June 2016, Pages 189–193