Enhancement of human mesenchymal stem cell infiltration into the electrospun poly(lactic-co-glycolic acid) scaffold by fluid shear stress

• hMSCs migrated along the direction of the fluid shear stress.
• hMSCs were infiltrated into PLGA scaffold by flow but PLGA micro-particles were not.
• Mechanotaxis enhanced hMSC infiltration into PLGA scaffold.

The infiltration of the cells into the scaffolds is important phenomenon to give them good biocompatibility and even biodegradability. Fluid shear stress is one of the candidates for the infiltration of cells into scaffolds. Here we investigated the directional migration of human mesenchymal stem cells and infiltration into PLGA scaffold by fluid shear stress. The human mesenchymal stem cells showed directional migrations following the direction of the flow (8, 16 dyne/cm2). In the scaffold models, the fluid shear stress (8 dyne/cm2) enhanced the infiltration of cells but did not influence on the infiltration of Poly(lactic-co-glycolic acid) particles.