کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1928547 | 1050371 | 2014 | 7 صفحه PDF | دانلود رایگان |
• C. sinensis-derived CsVAL peptide inhibits the degranulation of mast cells.
• CsVAL peptide inhibits FcεRI-mediated signal transduction in antigen-stimulated mast cell.
• CsVAL peptide prevents ROS generation in antigen-stimulated mast cells.
• CsVAL peptide inhibits the chronic contact hypersensitivity response.
Previous studies demonstrated that Clonochis sinensis-derived crude antigens suppress development of allergic responses. We investigated the effects of C. sinensis venom allergen-like (CsVAL) proteins on immune-modulating activities in allergic inflammatory response. Using RBL-2H3 rat mast cells, we demonstrated that CsVAL inhibits antigen-induced β-hexosaminidase release from immunoglobulin E-sensitized RBL-2H3 cells, and this inhibitory activity occurs by suppressing Lyn phosphorylation and intracellular reactive oxygen species production. In addition, CsVAL peptide treatment inhibits activation of protein kinase C-α and extracellular signal-regulated kinase 1/2, which are involved in degranulation of immunoglobulin E-sensitized mast cells. Furthermore, immunization with CsVAL suppressed development of skin inflammation by assessing ear thickness and cutaneous infiltration by eosinophils and mast cells in oxazolone-induced contact hypersensitivity in vivo mouse model. These results suggest that CsVAL is a promising candidate as an effective mast cell inhibitor for allergic and inflammatory diseases.
Journal: Biochemical and Biophysical Research Communications - Volume 445, Issue 3, 14 March 2014, Pages 549–555