کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1928627 | 1050380 | 2014 | 5 صفحه PDF | دانلود رایگان |

• We examined the level of miR-490-3p in A549 lung cancer cells compared with normal bronchial epithelial cell line.
• We are the first to show the function of miR-490-3p in A549 lung cancer cells.
• We demonstrate CCND1 may be one of the targets of miR-490-3p.
MicroRNAs (miRNAs) are small non-coding RNAs that negatively regulate the translation of messenger RNAs by binding their 3′-untranslated region (3′UTR). In this study, we found that miR-490-3p is significantly down-regulated in A549 lung cancer cells compared with the normal bronchial epithelial cell line. To better characterize the role of miR-490-3p in A549 cells, we performed a gain-of-function analysis by transfecting the A549 cells with chemically synthesized miR-490-3P mimics. Overexpression of miR-490-3P evidently inhibits cell proliferation via G1-phase arrest. We also found that forced expression of miR-490-3P decreased both mRNA and protein levels of CCND1, which plays a key role in G1/S phase transition. In addition, the dual-luciferase reporter assays indicated that miR-490-3P directly targets CCND1 through binding its 3′UTR. These findings indicated miR-490-3P could be a potential suppressor of cellular proliferation.
Journal: Biochemical and Biophysical Research Communications - Volume 444, Issue 1, 31 January 2014, Pages 104–108