کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1928923 | 1050433 | 2013 | 7 صفحه PDF | دانلود رایگان |
Poly(ADP-ribose) polymerase-2 (PARP-2) catalyzes poly(ADP-ribosyl)ation (PARylation) and regulates numerous nuclear processes, including transcription. Depletion of PARP-2 alters the activity of transcription factors and global gene expression. However, the molecular action of how PARP-2 controls the transcription of target promoters remains unclear. Here we report that PARP-2 possesses transcriptional repression activity independently of its enzymatic activity. PARP-2 interacts and recruits histone deacetylases HDAC5 and HDAC7, and histone methyltransferase G9a to the promoters of cell cycle-related genes, generating repressive chromatin signatures. Our findings propose a novel mechanism of PARP-2 in transcriptional regulation involving specific protein–protein interactions and highlight the importance of PARP-2 in the regulation of cell cycle progression.
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► PARP-2 acts as a transcription co-repressor independently of PARylation activity.
► PARP-2 recruits HDAC5, 7, and G9a and generates repressive chromatin.
► PARP-2 is recruited to the c-MYC promoter by DNA-binding factor YY1.
► PARP-2 represses cell cycle-related genes and alters cell cycle progression.
Journal: Biochemical and Biophysical Research Communications - Volume 431, Issue 1, 1 February 2013, Pages 58–64