کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1929069 1050443 2012 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Circadian rhythms and food anticipatory behavior in Wfs1-deficient mice
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Circadian rhythms and food anticipatory behavior in Wfs1-deficient mice
چکیده انگلیسی

The dorsomedial hypothalamic nucleus (DMH) has been proposed as a candidate for the neural substrate of a food-entrainable oscillator. The existence of a food-entrainable oscillator in the mammalian nervous system was inferred previously from restricted feeding-induced behavioral rhythmicity in rodents with suprachiasmatic nucleus lesions. In the present study, we have characterized the circadian rhythmicity of behavior in Wfs1-deficient mice during ad libitum and restricted feeding. Based on the expression of Wfs1 protein in the DMH it was hypothesized that Wfs1-deficient mice will display reduced or otherwise altered food anticipatory activity. Wfs1 immunoreactivity in DMH was found almost exclusively in the compact part. Restricted feeding induced c-Fos immunoreactivity primarily in the ventral and lateral aspects of DMH and it was similar in both genotypes. Wfs1-deficiency resulted in significantly lower body weight and reduced wheel-running activity. Circadian rhythmicity of behavior was normal in Wfs1-deficient mice under ad libitum feeding apart from elongated free-running period in constant light. The amount of food anticipatory activity induced by restricted feeding was not significantly different between the genotypes. Present results indicate that the effects of Wfs1-deficiency on behavioral rhythmicity are subtle suggesting that Wfs1 is not a major player in the neural networks responsible for circadian rhythmicity of behavior.


► Wfs1 immunoreactivity in DMH is almost exclusively found in the compact part.
► Low colocalization of Wfs1 and c-Fos in DMH during restricted feeding.
► Wfs1-deficiency results in lower body weight and reduced wheel-running activity.
► Circadian rhythmicity of behavior is mostly normal in Wfs1-deficient mice.
► FAA is normal in Wfs1-deficient mice.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 424, Issue 4, 10 August 2012, Pages 717–723
نویسندگان
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